Antioxidant properties and inhibitory effects of Satureja khozestanica essential oil on LDL oxidation induced-CuSO(4) in vitro

<p><b>OBJECTIVE</b>To assess various antioxidative activities of Satureja khozestanica essential oil (SKE) and its effect on oxidation of low density lipoprotein (LDL) induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde (MDA).</p><p><b>METHODS</b>The formation of conjugated dienes, lag time and MDA were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE. Also total antioxidant activity and free radical scavenging of SKE were determinated.</p><p><b>RESULTS</b>It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation. The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200 µg/mL. Total antioxidant capacity of SKE was (3.20±0.40) nmol of ascorbic acid equivalents/g SKE. The SKE showed remarkable scavenging activity on 2, 2-diphenyl-picrylhydrazyl, IC50 (5.30±0.11) ng/mL.</p><p><b>CONCLUSIONS</b>This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.</p>

Evaluation of radiolabeled streptokinase for thrombosis imaging

Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. In this work a recently developed radiolabeled streptokinase [STP] tracer was evaluated in an animal thrombotic model using SPECT imaging and biodistribution studies. Locally labeled [67Ga]-Streptokinase was checked by ITLC, HPLC and SDS-PAGE experiments to check the tracer integrity and purity. The biodistribution studies were performed in thrombotic femoral vein of rats using tissue counting and preliminary SPECT studies, respectively [up to 2 h]. [67Ga]-Streptokinase prepared with suitable radiochemical purity [HPLC >95%, ITLC >99%] was administered to FeCl3 induced thrombotic rats and the percentage of injected dose per gram of tissue [ID/g%] as well as the SPECT images demonstrated the good specific binding of the tracer in thrombotic clots located in heart and aorta 2 hours post injection. [67Ga]-DTPA-STP can be a suitable probe for imaging of thrombosis in cardiovascular diseases. Ga-68 labeled STP has the potential to be an alternative superior labeled compound due to positron emission properties for PET studies as well as appropriate physical half life